<th id="6a00e"></th>
  • 
    
  • Changes in α-Farnesene and Phenolic Metabolism and the Expression of Associated Genes during the Development of Superficial Scald in Two Distinct Pear Cultivars

    Author:Jingang He , Yunxiao Feng , Yudou Cheng , Thirupathi Karuppanapandian , Jinxiao Wang and Junfeng Guan
    Published:2020
    Abstract:

    Superficial scald is a postharvest physiological disorder that occurs in pear during and
          after cold storage. In this study, the superficial scald index; α-farnesene and its oxidation products,
          conjugated trienols (CTols); phenolic content; and the expression of its related genes were investigated
          in two different pear cultivars, ‘Wujiuxiang’ (Pyrus communis L.) and ‘Yali’ (Pyrus bretschneideri R.),
          following 115 days of cold storage at 0 ?C followed by 7 days of shelf life at 20 ?C. The results
          indicated that the superficial scald occurred after 115 days of cold storage and became more severe
          during the shelf life of the ‘Wujiuxiang’ pear, whereas no scald was observed in ‘Yali’. The α-farnesene
          levels increased rapidly at first and then decreased, while the CTols contents increased significantly
          in ‘Wujiuxiang’ as compared to ‘Yali’, and the expression levels of the genes involved in α-farnesene
          and CTols metabolism (HMGR1, HMGR2, GSTU7, GPX5, and GPX6), as well as the phenolic synthesis (PAL1, PAL2, C4H1, 4CL2, C3H, and ANR) of the peel, were significantly up-regulated at the
          onset of the superficial scald. In addition, the relative conductivity and contents of catechin and
          epicatechin were higher, and the expression level of the laccase gene (LAC7) significantly increased
          with the development of superficial scald, while lower contents of chlorogenic acid, arbutin, and
          isorhamnetin-3-3-glucoside, as well as the lower expression levels of a phenolic-synthesis-related
          gene (C4H3) and polyphenol oxidase genes (PPO1 and PPO5), were noticed in ‘Wujiuxiang’ as
          compared to ‘Yali’. The results indicated that the onset and progression of superficial scald were associated with the accumulation of CTols, cell membrane breakdown, and higher catechin, epicatechin,
          and rutin contents, as well as the expression of associated genes of the peels of pear fruit.

    Key words:pear; α-farnesene; conjugated trienols; phenolics; superficial scald

     
    久久久欧美亚洲精品_欧美日韩精品不卡在线观看_亚洲失禁一区二区_凹凸国产熟女精品视频国语_秋霞电影网院午夜理论片免费看